A polymerase chain reaction (PCR) tool has been developed by Q Chip to speed up bacterial identification with greater accuracy and less variability. Identification using its ReaX Assay 16S beads is based on PCR amplification of the widely-used 16S ribosomal ribonucleic acid gene.
In a comparative study of the identification of E. coli and S. aureus, the ReaX Assay 16S beads generated identical PCR bands for each triplicate and for each organism, compared with conventional liquid-based PCR methods. However, with the ReaX Assay 16S PCR beads, less manual pipetting was required, with fewer opportunities for contamination.
Subsequent sequence analysis of the amplification products of both organisms revealed that the ReaX Assay 16S beads gave excellent DNA sequence detection results, demonstrating that this enhanced PCR reagent formulation is an excellent complement to post-PCR applications.
PCR reaction set-up using the ReaX Assay 16S beads requires no prior optimisation and is quicker and more user-friendly to perform compared to conventional liquid-based PCR.
ReaX Assay 16S beads contain all the reagents required to perform 16S ribosomal ribonucleic acid gene amplification.
Contact: Q Chip; Tel: 029 2068 2092