Microbiology specialist Lab M has introduced a new medium for the enumeration of presumptive E. coli in milk and milk products. Its modified lauryl sulphate tryptose broth adds 4-methyl- umbelliferyl-?-D-glucuronide (MUG) to the standard lauryl tryptose broth. This allows presumptive E. coli to be enumerated from presumptive coliforms in milk and milk products.
Used in conjunction with the most probable numbers technique, the broth is used as a confirmatory test for lactose fermentation and gas production for presumptive coliforms. The addition of MUG to the medium allows the positive discrimination of E. coli strains. The majority of E. coli harbour the glucuronidase enzyme and release a fluorogenic compound. Test tubes which fluoresce under ultra-violet light are then confirmed for E. coli with Kovac's reagent.
Contact: Lab M; Tel: 0161 797 5729